Effects of individual or combined use of alpha-lipoic acid and methylprednisolone on malondialdehyde, superoxide dismutase, and catalase levels in acute spinal cord injury in rats

  • Alper Birgul Kilis State Hospital, Department of Neurosurgery, Kilis – TURKEY
  • Gokhan Canaz Bakirkoy Research and Training Hospital for Neurology, Neurosurgery and Psychiatry
  • Murat Ugur Kilis State Hospital, Department of Neurosurgery, Kilis – TURKEY
  • Mehmet Tokmak Medipol University, Department of Neurosurgery, Istabul – TURKEY
  • Metin Kasap Haseki Training and Research Hospital, Department of Neurosurgery, Istanbul – TURKEY
  • Ibrahim Alatas Sisli Florence Nightingale Hospital, Istanbul Bilim University, Department of Neurosurgery, Istanbul – TURKEY
  • Huseyin Canaz Sisli Florence Nightingale Hospital, Istanbul Bilim University, Department of Neurosurgery, Istanbul – TURKEY
Keywords: Alpha Lipoic Acid, Methylprednisolone, Malonyldialdehyde, Superoxide Dismutase, Spinal Cord Injury

Abstract

Introduction: Recent studies have demonstrated that alpha-lipoic acid (ALA) has a strong antioxidant property and it exerts neurotrophic effects on the peripheral nerves. In this study, we investigated potential effects of ALA on secondary injury mechanisms as well as on apoptosis.
Methods: Forty Sprague-Dawley rats were equally divided into 5 groups, as follows: laminectomy (control), laminectomy + trauma (Trauma), laminectomy + posttraumatic methylprednisolone [MP] (Trauma + MP), laminectomy + posttraumatic ALA (Trauma + ALA), laminectomy + posttraumatic MP and ALA (Trauma + MP + ALA). Yasargil aneurysm clip method was used to induce the spinal cord injury. Twenty-four hours after the procedure the rats were sacrificed. Spinal cord samples were harvested to analyze malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels, as well as histopathological characteristics.
Results: The Kruskal-Wallis test (95% confidence level, p < 0.05) showed a statistically significant difference between the groups in MDA (p = 0.006), CAT (p = 0.000), and SOD (p = 0.001) levels. Pairwise comparisons, with Bonferroni correction, of control and Trauma group with the other groups, revealed a significant difference in CAT and SOD levels. Overall, our results showed that ALA administration significantly decreased MDA levels in Trauma + ALA compared to the same effect of MP in Trauma + MP group. Furthermore, ALA administration increased SOD and CAT levels in Trauma + ALA group. The combined use of ALA and MP demonstrated synergistic effects and yielded even more significant results.
Conclusions: A combined use of ALA and MP might provide a benefit in patients exposed to spinal cord injury. However, at present, further studies are required to confirm these results.

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Published
2016-12-20
How to Cite
Birgul, A., Canaz, G., Ugur, M., Tokmak, M., Kasap, M., Alatas, I., & Canaz, H. (2016). Effects of individual or combined use of alpha-lipoic acid and methylprednisolone on malondialdehyde, superoxide dismutase, and catalase levels in acute spinal cord injury in rats. Journal of Health Sciences, 6(3), 174-183. https://doi.org/10.17532/jhsci.2016.409
Section
Research articles