Comparison of ARCHITECT chemiluminiscent microparticle immunoassay for determination of Troponin I in serum with AXYM MEIA technology

Authors

  • Nafija Serdarević Institute for Clinical Chemistry and Biochemistry, University of Sarajevo Clinics Center; Faculty of health sciences, Bolnička 25, 71000 Sarajevo, Bosnia and Herzegovina

DOI:

https://doi.org/10.17532/jhsci.2011.130

Keywords:

Troponin, MEIA and CMIA

Abstract

Introduction: The aim of this study was determination of troponin I at serum using Architect (Abbott) and AxSYM System (Abbott). Troponin is regulatory subunit of the troponin complex associate with actin filament within muscle cells and it is a marker for diagnosis of myocardial damage.
Methods: We used Architect STAT chemiluminescent microparticle immunoassay (CMIA) and AxSYM microparticle Enzyme Immunoassay (MEIA), techniques for quantitative determination of cardiac TnI in human serum or plasma. At our study we have proved precision, reproducibility and accuracy from both methods. The investigation included patients (n=119) who have myocardial infarction or ischemic heart damage and were treated at cardiology, emergency, internal medicine and neurology unit in Clinical Center University in Sarajevo.
Results: The precision for three controls using Architect STAT TnI asssay technology were 3.6 – 5.2 % and reproducibility was 3.7 to 5.6 %. The AxSYM STAT TnI has precision for three controls 4.3–6.6 % and reproducibility
was from 4.8 to 7.8 %. We have got very good correlation between Architect and AxSYM technology r = 0.999 in the investigation of troponin I in serum.
Conclusions: We can conclude that chemiluminescent troponin assay I (Architect) showed good analytical performance and gave new possibility at troponin I determination.

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Published

15-12-2011

How to Cite

1.
Serdarević N. Comparison of ARCHITECT chemiluminiscent microparticle immunoassay for determination of Troponin I in serum with AXYM MEIA technology. JHSCI [Internet]. 2011 Dec. 15 [cited 2024 Mar. 29];1(3):154-8. Available from: https://www.jhsci.ba/ojs/index.php/jhsci/article/view/38

Issue

Section

Research articles