Performance evaluation of three laboratory diagnostic methods for intestinal parasitic infections at rural Bahir Dar, Northwest Ethiopia: a cross-sectional study

Authors

  • Birtukan Bayayibign Felege Hiwot Referral Specialized Hospital, Medical Laboratory Service, Bahir Dar, Ethiopia
  • Mulat Melaku Department of Medical Laboratory Sciences, School of Health Sciences, College of Medicine and Health Science, Bahir Dar University, Ethiopia https://orcid.org/0000-0001-5840-513X
  • Woynshet Gelaye Department of Medical Laboratory Sciences, School of Health Sciences, College of Medicine and Health Science, Bahir Dar University, Ethiopia

DOI:

https://doi.org/10.17532/jhsci.2019.170

Keywords:

wet mount, modified Baermann, Ritchie's Method, rural Bahir Dar, Ethiopia

Abstract

Introduction: Even if the prevalence of intestinal parasites is high in Ethiopia, we still use only direct wet mount method for laboratory diagnosis of intestinal parasitic infections, having low sensitivity, and this significantly increases false-negative results. Therefore, the performance evaluation of three laboratory diagnostic methods is mandatory. 

Methods: Single stool sample was collected from March 2018 to June 2018, among 211 school children, and processed using a wet mount, modified Baermann (MB), and Ritchie’s methods. The sensitivity and negative predictive values (NPVs) at 95% confidence interval and Kappa values were calculated in terms of the gold standard method (the combined result of altogether). 

Results: The overall prevalence of intestinal parasites was 60.2%. The sensitivity and NPVs of the wet mount, MB, and Ritchie’s methods against the “Gold standard” test were 49.6% and 56.8%, 80.3% and 77.1%, and 67.7% and 68.8%, respectively. 

Conclusions: MB showed the best, and wet mount showed the least performance for the laboratory diagnosis of intestinal parasitic infections.


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Performance evaluation of three laboratory diagnostic methods for intestinal parasitic infections at rural Bahir Dar, Northwest Ethiopia: a cross-sectional study

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Published

01.02.2019

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Research articles

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How to Cite

1.
Performance evaluation of three laboratory diagnostic methods for intestinal parasitic infections at rural Bahir Dar, Northwest Ethiopia: a cross-sectional study. JHSCI [Internet]. 2019 Feb. 1 [cited 2024 Jun. 18];9(1):34-9. Available from: https://www.jhsci.ba/ojs/index.php/jhsci/article/view/170