Evaluation of tumor marker HE4 assay on the Elecsys 2010 analyzer

  • Jozo Ćorić Department of Clinical Chemistry, Clinical Center of Sarajevo University, Bolnička 25, 71000 Sarajevo, Bosnia and Herzegovina
  • Lejla Hasanbegović Department of Clinical Chemistry, Clinical Center of Sarajevo University, Bolnička 25, 71000 Sarajevo, Bosnia and Herzegovina
  • Aleksandar Bodulović Department of Clinical Chemistry, Clinical Center of Sarajevo University, Bolnička 25, 71000 Sarajevo, Bosnia and Herzegovina
  • Jasminka Mujić Department of Clinical Chemistry, Clinical Center of Sarajevo University, Bolnička 25, 71000 Sarajevo, Bosnia and Herzegovina
Keywords: Human epididymis protein 4 (HE4), epithelial ovarian cancer, biomarkers

Abstract

Introduction: Whey-acidic protein human epididymis protein 4 (HE4) is a new promising biomarker for epithelial ovarian cancer. The measured HE4 values may depend on the testing procedure used. The aim of this study was to evaluate the

Methods: We evaluated a HE4 method on Elecsys 2010 analyzer. The method for quantitative determination of HE4 is direct, competitive chemiluminescent immunoassay. For quality control we use Elecsys PreciControl HE4 1 and 2. HE4 was measure on sera obtained from 56 women ( 20 healthy and 36 with epithelial ovarian cancer).

Results: The Roche HE4 assays showed a good linearity (r=0.99) and precision (intrassayed total CV<5%). The median HE4 serum concentrations was significantly higher among EOC patients than healthy females (p<0,05). Elevated levels HE4 were found in 78 % patients with epithelial ovarian cancer.

Conclusions: The presented results of the analytical evaluation methods for the determination of HE4 on the Elecsys 2010 analyzer showed an acceptable accuracy and precision.

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Published
2014-04-15
How to Cite
Ćorić, J., Hasanbegović, L., Bodulović, A., & Mujić, J. (2014). Evaluation of tumor marker HE4 assay on the Elecsys 2010 analyzer. Journal of Health Sciences, 4(1), 36-39. https://doi.org/10.17532/jhsci.2014.148
Section
Research articles